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Image Search Results
Journal: Oncotarget
Article Title: Identification of neoepitopes recognized by tumor-infiltrating lymphocytes (TILs) from patients with glioma
doi: 10.18632/oncotarget.24955
Figure Lengend Snippet: TILs from five patients with GBM were exposed to mutated peptides predicted to be private tumor-specific epitopes, along with the corresponding wildtype sequences. After seven days of culture, supernatants were harvested for measurement of IFN-γ, TNF-α and IL-17A by sandwich ELISA. Based on the actual concentration of cytokines produced by TILs co-cultured with the individual wildtype or mutated peptides, heat maps were created depicting the peptide-induced responses unique to each patient. A scale ranging from low (black) to high (red) amounts of cytokine produced by total TILs, the CD4+ as well as the CD8+ TIL subsets are shown. Also shown in the scale are cytokine concentration values ranging from 0–4804 pg/10e5 TIL/days. The identical gene name appears several times for some patients due to recognition of different epitopes/peptides belonging to the same gene product. Actual cytokine concentrations are provided in .
Article Snippet: Cultures of CD4+ and CD8+ TILs with the individual peptides were performed after separation of the T-cell subsets from total TIL cultures using the
Techniques: Sandwich ELISA, Concentration Assay, Produced, Cell Culture
Journal: Oncotarget
Article Title: Identification of neoepitopes recognized by tumor-infiltrating lymphocytes (TILs) from patients with glioma
doi: 10.18632/oncotarget.24955
Figure Lengend Snippet: Number of mutations that were recognized by TILs/number of mutations identified by whole DNA exome sequencing
Article Snippet: Cultures of CD4+ and CD8+ TILs with the individual peptides were performed after separation of the T-cell subsets from total TIL cultures using the
Techniques:
Journal: BioMedical Engineering OnLine
Article Title: Mechanically strained osteocyte-derived exosomes contained miR-3110-5p and miR-3058-3p and promoted osteoblastic differentiation
doi: 10.1186/s12938-024-01237-9
Figure Lengend Snippet: Levels of IGF-1 protein and PGE2 in the culture supernatant of MLO-Y4 osteocytes, and NOS activity in MLO-Y4 osteocytes. The ELISA result indicated that the mechanical tensile strain increased the IGF-1 and PGE2 levels of MLO-Y4 osteocytes ( A ), and increased NOS activity of the osteocytes, the NOS activity was determined via a colorimetric method ( B ). n = 6, * P < 0.05, ** P < 0.01, between the indicated groups
Article Snippet: After mechanical stimulation, the levels of PGE2 hormone and IGF-1 protein in the culture supernatant of MLO-Y4 cells were assayed separately using a
Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay
Journal: BioMedical Engineering OnLine
Article Title: Mechanically strained osteocyte-derived exosomes contained miR-3110-5p and miR-3058-3p and promoted osteoblastic differentiation
doi: 10.1186/s12938-024-01237-9
Figure Lengend Snippet: ALP activity, and the levels of BMP-2, Col-I and Runx2 in MC3T3-E1 osteoblasts. The mechanical tensile strain heightened ALP activity of MC3T3-E1 osteoblasts ( A ), up-regulated Runx2 mRNA expression in osteoblasts (as shown by RT‒qPCR) ( B ), increased BMP-2 protein level in medium of osteoblasts (as shown by ELISA) ( C ), and up-regulated protein levels of Collagen type I (as shown by Western blot) ( D ). n = 5, * P < 0.05, ** P < 0.01, between indicated groups. ALP activity was assayed with a colorimetric method
Article Snippet: After mechanical stimulation, the levels of PGE2 hormone and IGF-1 protein in the culture supernatant of MLO-Y4 cells were assayed separately using a
Techniques: Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot